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JobsThermo Fisher

qPCR Scientist III, Molecular Biology

Thermo Fisher

qPCR Scientist III, Molecular Biology

Thermo Fisher

Singapore, Singapore

·

On-site

·

Full-time

·

2w ago

Required Skills

qPCR

Molecular biology

Assay development

Assay validation

Data analysis

Nucleic acid extraction

cDNA synthesis

Mentoring

Work Schedule
Standard (Mon-Fri)

Environmental Conditions
Cold Room/Freezers -22degreesF/-6degrees C, Laboratory Setting, Office, Some degree of PPE (Personal Protective Equipment) required (safety glasses, gowning, gloves, lab coat, ear plugs etc.)

  • Job Description Key Responsibilities

  • Lead the design, optimization, and validation of qPCR assays for gene expression, genotyping, and molecular diagnostics applications.

  • Perform RNA/DNA extraction, cDNA synthesis, and nucleic acid quantification using spectrophotometric and fluorometric methods.

  • Validate assay performance, including sensitivity, specificity, efficiency, and reproducibility.

  • Analyze and interpret qPCR data using software such as Applied Biosystems Quant Studio, Bio-Rad CFX Manager, or equivalent platforms.

  • Troubleshoot technical issues and propose innovative solutions to improve assay performance.

  • Maintain accurate and detailed experimental records, laboratory notebooks, and data reports.

  • Collaborate cross-functionally with R&D, QC, and bioinformatics teams to support product development or experimental studies.

  • Ensure compliance with laboratory safety protocols, quality management systems, and regulatory standards (e.g., ISO, GLP, or CLIA, as applicable).

  • Present experimental findings in technical meetings and contribute to scientific documentation and publications.

  • Mentor junior scientists and research associates, providing technical guidance and fostering a culture of collaboration and continuous improvement.

**Qualifications Education: **Master’s or Ph.D. in Molecular Biology, Biochemistry, Genetics, or a related life sciences discipline. Experience requirements will vary based on degree.

Experience:

  • Minimum of 2–5 years of hands-on experience performing qPCR (probe based chemistry preferred), including multiplexed qPCR reactions.

  • Prior experience with assay development, validation, and data analysis.

  • Familiarity with nucleic acid extraction instruments or workflows is expected.

  • Additional experience in NGS is a plus.

Technical Skills:

  • In-depth understanding of molecular biology principles, qPCR kinetics, and assay troubleshooting.

  • Strong analytical and problem-solving skills with a data-driven mindset.

  • Strong understanding of qPCR principles, primer/probe design, and amplification kinetics.

  • Proficiency in data analysis, statistical interpretation, and report generation.

  • Experience working under quality and regulatory standards preferred.

Soft Skills:

  • Excellent organizational, communication, and problem-solving skills.

  • Ability to work independently as well as collaboratively in a multidisciplinary environment.

  • Strong attention to detail and scientific curiosity.

  • Preferred Qualifications

  • Familiarity with bioinformatics tools for assay design

  • Experience with digital PCR (dPCR)

  • Knowledge of automation and data management systems.

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About Thermo Fisher

Thermo Fisher

Thermo Fisher Scientific Inc. is an American life science and clinical research company. It is a global supplier of analytical instruments, clinical development solutions, specialty diagnostics, laboratory, pharmaceutical and biotechnology services.

10,001+

Employees

Waltham

Headquarters

Reviews

3.9

2 reviews

Work Life Balance

3.5

Compensation

2.5

Culture

3.5

Career

4.0

Management

3.0

65%

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Pros

Large company with extensive resources

Structured internship program

Career opportunities in product management

Cons

Low compensation ($22/hour)

High cost of living in location

Expensive housing/rent

Salary Ranges

2,260 data points

Mid/L4

Mid/L4 · Adobe Analytics Launch Developer

1 reports

$137,796

total / year

Base

$105,997

Stock

-

Bonus

-

$137,796

$137,796

Interview Experience

8 interviews

Difficulty

3.0

/ 5

Duration

14-28 weeks

Offer Rate

12%

Experience

Positive 12%

Neutral 63%

Negative 25%

Interview Process

1

Application Review

2

Recruiter Screen

3

Hiring Manager Interview

4

Panel Interview

5

Final Interview

6

Offer

Common Questions

Technical Knowledge

Behavioral/STAR

Past Experience

Culture Fit

Industry Specific